Ancient Unexplained Files (2021–…): Season 1, Episode 6 - Gladiator Graveyard - full transcript

Experts reveal the mystery behind a macabre scene of ancient execution.

[narrator] digital archeology

Reveals the true origins of machu picchu.

It's like trying to build manhattan

In the middle of the rockies.

It just doesn't make any sense.

[narrator] molecular science

Resolves a neolithic apocalypse.

It's one of the most significant
sites in human history.

What happened to this settlement
that was wiped out in a heartbeat?

[narrator] high-tech lasers

Trace the owners of a
stash worth millions of dollars.



This is real-life buried treasure.

It's as if they had found
the tomb of tutankhamun

On their island. I
mean, it's that big a deal.

[narrator] in a digital-technology
lab of the future,

Experts uncover clues
invisible to the naked eye.

I've never seen anything like that before.

[narrator] and solve mysteries

That have baffled for centuries.

That, right there, is what
scientists have been missing.

All the evidence points
to an incredible discovery.

This could be a game changer.

[narrator] state-of-the-art
digital technology,

Closes the toughest cases.

This mystery finally makes sense.



[narrator] from the
ancient unexplained files.

[narrator] york, England.

2004, a shallow mass
grave of mutilated bodies

Is unearthed beneath
a residential backyard.

They find a few bones
representing an individual.

And then more and more.

In total, they find 82 individuals.

[yeomans] this is a very grisly discovery.

It's clear that these individuals

Were subjected to
some terrible experiences.

Nearly three-quarters
of them were decapitated.

And many of the rest
had their skulls crushed.

[narrator] in the midst
of this bone wreckage

Is a skeletal wound, oddly out of place.

One of these skeletons
has a series of depressions

In the pelvic bones, right above the hips.

These injuries weren't
caused by any weapon.

It looks like the bite mark
of something big and scary.

It's baffling.

[narrator] now, can
cutting-edge digital analysis,

Solve the cause of this puncture wound,

Along with the rest of this
macabre human bone yard?

[auerbach] it's a grisly scene.

But the bones are a
treasure trove of clues.

Who are the victims of this grave?

[narrator] york archaeological
trust excavations

Take over a year.

The evidence of violence is astonishing.

Right out of the ground,
it's clear these skeletons

Have suffered some horrific neck injuries.

But they're very clean
injuries to the spinal column.

They're very likely to have
been made by a sword or an axe.

Many of the skulls have been cracked open.

And show evidence of
being hit with a hammer

Or perhaps the blunt end of a blade.

[narrator] to determine
when this butchery took place,

Bone fragments are sent for analysis.

Radiocarbon dating tells
us these bones are very old.

This isn't a modern day crime scene.

Nor is it a medieval one.

The bones are 1800 years old.

And they date back to roman times.

[narrator] in 71 ce,

The romans establish
york and name it eboracum.

This vital provincial
capital and military base

Supports the empire's
push north towards scotland.

York started off life
as a leisurely fortress.

And then becomes one of the leading cities

In roman britain.

And the largest city in northern britain,

And controlling a vast area of territory.

And would've been a very
flourishing, important place.

[narrator] by 306 ce,

The city's strategic value
led to constantine the great,

Being acclaimed resident emperor.

It had lively markets,

Baths, theaters, temples.

It was a very characteristic
roman city for 300 years.

[narrator] to maintain an iron
grip over their new territory

The romans utilized capital punishment.

High crimes are dealt
increasingly gory executions.

So, is it possible that
we're looking at a site

Of a mass execution of criminals?

It's well documented that
romans' methods of punishment

Were often quite brutal.

[auerbach] those sentenced
to die could be killed

In any number of ways.

They could be drowned.

They could be buried alive.

They could be cast from a great height.

They could be cast to
beasts to be devoured.

They could be crucified.

Or they could be decapitated.

It's possible this is a
roman execution cemetery.

And these bodies were beheaded.

Maybe that was the method of death.

[narrator] but when investigators
study the bodies anatomically,

A potential flaw arises.

[maca] if this were an execution cemetery,

You would expect to see there represented

A broad cross-section of society.

But this clearly isn't the case.

Analysis of the 82 individuals

Shows that all of them
were under the age of 45

And the vast majority were males.

[narrator] more curious,

They appear to be an elite force.

They're a bit larger than
the normal population.

So, these relatively well fed individuals.

Those who've gained a greater stature.

[valentine] the muscle attachments tell
us that these were big, muscular men.

But they were only really
developed on one side.

And this is consistent with

Very heavy weapons
training from a young age.

It's possible we could be looking

At a grave of roman soldiers.

[narrator] york is home to two legions.

The largest military
unit of the roman army.

One of the legendary vi legion victrix,

Who helped save cleopatra
in the siege of alexandria.

In this period, the romans
are constantly looking

To expand their territory.

And they were pushing the boundaries

Of the northern empire Well into scotland.

And, so, this puts the roman
armies on a collision course

With the fierce caledonian tribes.

[narrator] it's reckoned
around 50,000 roman soldiers

Are casualties of the
brutal war in the north.

[yeomans] so we would expect

To find the graves or the
remains of roman soldiers

Who were killed in
these very intense battles.

It's possible that this
grave could represent

Their final resting place.

[narrator] but, as experts
sift through bone injuries,

A strange set of puncture
wounds in a pelvis

Gives them pause for thought.

They're unlike anything
found from the battle field.

The pelvis is converted
into a virtual model.

The digital forensics lab

Allows bio medic greg szulgit,

Access to cutting-edge tech-analysis.

[szulgit] okay, we can clearly see that

This appears to be a bite mark

From some kind of large vicious creature.

The giveaway is that these indentations

Mirror a row of incisors.

The fact that these have penetrated

Both flesh and solid bone

Indicates tremendous force

That only a powerful animal is capable of.

Now, this presents a bigger mystery.

What is this formidable beast?

Nothing in britain matches this profile.

Hmm, okay, what else can
we learn from the pelvis?

Well, there are a few
circular wounds up here.

And it looks like, maybe,

A set of canine teeth

May have clamped on to the hip.

If I measure the diameter,
it's about a quarter-inch.

If this large hole is from a canine tooth,

Then this is from an apex predator.

Something like a big cat.

Let's call up a standard lion's jaw.

What's great about digital tech

Is that we can have it
chomp on this pelvis.

Look at this!

The incisors line up.

And the canines fit almost perfectly.

But here's the rub.

Lions went extinct in britain

More than 12,000 years ago.

Millennia before this guy was born.

It makes no sense at all.

[narrator] a roman cemetery,

Found in historic york, England,

Has thrown experts into confusion.

Among a litany of
gruesome skeletal injuries

Is a bite mark,

Likely by a big cat predator.

But lions went extinct in britain
more than 12,000 years ago.

[narrator] investigators now
hope this skeletons' backgrounds

May shed some more light.

They test tooth sample
for chemical elements

Called isotopes.

[horton] the simplest is diet.

We are what we eat.

And it's possible to look at two different

Isotopic pathways of carbon

Which are known as c3 and c4.

If we live on a diet of wheat and barley

And so forth, we're predominantly c3.

But if we are eating
middle-eastern crops, for example,

We'll have more of a stronger c4 signature.

And a number of these
individuals seem to have

C4 diets.

Which suggests that they'd come from

North africa area.

What we have is a group of strong,

Capable fighting men,

Drawn from the four corners of the empire.

All are buried in the same place.

They all show signs of injury by weapons

Across the entire course of their lives.

And one of them

Appears to have been savagely injured

By a large carnivore.

Taken all together,

This evidence leads to only one conclusion.

These men were not soldiers.

They were gladiators.

[victorious scream]

[narrator] gladiators are
lauded across the roman empire

And hollywood blockbusters.

Men would engage in
deadly hand to hand combat

Or as bestiary Against apex predators.

[horton] you've got two groups of bestiary.

One group who are
essentially the criminals.

And they're just placed
into the arena naked.

With nothing.

And the crowd watched
them being pulled to death.

[lion roars]

And then, you've then got the professionals

Who do this as a job
with their given weapons.

And they undertake these conflicts.

[narrator] most feared are the big cats.

An account tells of 200 roman bestiary

Being mauled by a single lion.

[yeomans] some sources indicate

That gladiators who were
forced to fight as bestiary

Would often prefer suicide.

Because they knew that
it would be, very likely,

A painful and violent end.

It's possible that some of
the people in the cemetery

Were criminals involved
in these spectacles.

But what's interesting is that

They're all being buried
in the revered fashion,

In proper cemeteries

And the decapitation
is part of a burial ritual.

So, it does suggest that these were mostly

Professional combatants

Paid to perform in the
circuses in ancient york.

[narrator] digital tech and science

Have pieced together
these freelance warriors

Buried in a backyard.

But one crucial mystery

Still eludes experts to this day.

[horton] what intrigues
me as an archeologist

Is where is the amphitheater
where this happened?

Because, so far, we
haven't found it in york.

[narrator] abu hureyra, syria.

In 1972,

Archeologists uncovered the
scorched remnants of an ancient village.

It's a huge mound that once contained

Series of circular dwellings.

Experts suspect that it
dates back to the stone age.

But extensive deposits of charred remains

Show that there was a big
catastrophe there at some point.

[narrator] mysteriously, the
burn patterns seem way off

Any normal phenomenon.

[szulgit] evidence of some charred remains

Suggests extremely high temperatures,

Far higher than any inferno would achieve.

This is mind-blowing.

[narrator] now, a new
scientific investigation

Aims to crack this half-century puzzle.

Revealing an earth-shattering event,

[loud explosion]

That turned the course of civilization.

Archeologists rate abu hureyra

As one of the most significant sites

In human history.

These hunter-gatherers
were a resourceful bunch.

Adept at not only surviving, but thriving.

What happened to this
once prosperous settlement

That was wiped out in a heartbeat?

[explosion]

[narrator] the incinerated
site is about 75 miles

From the city of aleppo.

In the 1970s, dictator hafez al-assad

Rubber stamps a large dam

That will flood the euphrates valley

And sink abu hureyra.

[wright] with the prospect that
abu hureyra will be lost forever,

Archeologists rushed to excavate the site

And salvaged materials.

[szulgit] during the excavations,

Sediment is taken away in
layers from excavation trenches.

Scientists discovered large
quantities of charcoal deposits

In one of the bottom layers.

They radiocarbon date the charcoal

To find that it's 12,800 years old.

[narrator] back then, this
region was starkly different

From today's arid wasteland.

Stone age humans
feasted on a bountiful land.

Civilization is said to have
developed independently

At six different sites across the globe.

Two of these are in the middle east,

In a region known as the fertile crescent.

And at its heart sits
the site of abu hureyra.

This would have been an ideal location

For a band of
hunter-gatherers at this time.

The nearby euphrates river
would've provided fresh fish.

And plenty of gazelles in migration

Offering a constant supply of fresh meat.

[narrator] but the fundamental
question of what obliterated abu hureyra

Stumps '70s investigators.

Fifty years later,

A new team takes up the challenge

And makes a striking discovery.

To put it in context,

Those temperatures would
turn something like your car

Into a molten pool of
metal in under a minute.

[narrator] in syria, A 1970s investigation

Into why stone age abu
hureyra was wiped off the map

Has hit a brick wall.

Scorched remnants defy normal explanation.

Now, a new team is on the hunt for answers.

It's hoped that by studying
the sediment layers once more,

We'll get some clues as
to what may have caused

Abu hureyra's sudden demise.

[proctor] in a sample of the charcoal,

Scientists find miniature
fragments of melt glass.

Melt glass forms from
incredibly high temperatures.

Far higher than any
house fire could achieve.

This categorically rules
out fire from being the culprit.

[narrator] scientists analyze
the melt glass composition

At an elemental level.

Using an energy-dispersive
x-ray spectroscopy test.

[proctor] this cutting-edge
technique allows scientists

To hit a sample with an electron beam

And measure the x-rays emitted.

This process reveals the
elements present in the sample.

[szulgit] the results show that the melt
glass contains quartz, zircon and chromite,

All of which have extremely
high melting temperatures.

[narrator] to determine
exactly what the temperature is,

Technicians fire up the lab furnaces

To fry the samples.

It's only when the lab furnaces max out

At 3,400 degrees fahrenheit,

That the scientists see
similar melt glass being made

As is found in the site at abu hureyra.

To put it in context,

Those temperatures would
turn something like your car

Into a molten pool of
metal in under a minute.

[narrator] by comparison,

Volcanic lava only reaches 2,200 degrees.

To generate the extraordinary
heat needed to create this melt glass,

An explosion of nuclear force is required.

Back in the stone age, one of the
few possible sources of that energy

Is a comet, meteor or asteroid.

[explosion]

[narrator] in 2017, researchers
find an asteroid strike in canada

Generated record temperatures
of over 4,300 degrees

Producing melt glass like abu hureyra.

But one potential flaw remains.

Surveys of abu hureyra
show no sign of a crater.

So one possible explanation

Is a comet or meteor
exploding in our atmosphere,

Otherwise known as an airburst.

[loud explosion]

When a comet enters the
earth's upper atmosphere

The rise and pressure in temperature

Can cause it to break up into pieces.

Each piece can generate a fireball
with almost a megaton of energy.

[loud explosion]

[narrator] scientists switched their focus

To finding irrefutable airburst evidence.

One telltale signature Is nanodiamonds.

Nanodiamonds are formed
under immense heat and pressure

From a cosmic impact.

They are ridiculously small.

You could fit more than 20 of them

On a single covid-19 viron.

So, finding them is like
a needle in a haystack.

[narrator] a tiny sediment
sample is doused in five acids.

Only nanodiamonds can
withstand this corrosive cocktail.

Essentially, this method
burns down the haystack

To find the needle.

[narrator] then a high-powered
electron microscope

Confirms their presence.

Proving, around 11,000 bce

Abu hureyra was instantly incinerated

By an airburst.

[explosion]

Evidence shows that these hunter-gatherers

Were really doing very well
with their chosen environment.

Microwear indicates stone tools

Were used to butcher
animals and to skin their hides.

Until, all of a sudden, these
hunter-gatherers would've seen

Just a gigantic flash and then

They would've been vaporized
along with everything around them.

[narrator] abu hureyra fell victim to
a devastating paleolithic catastrophe,

Known as the younger dryas impact.

Huge pieces of a two-mile wide comet

Blaze destruction from the
middle east to the americas.

The range of the younger
dryas boundary impact

Is mind-boggling.

Wildfires might have incinerated up to

Ten percent of all the
forested areas on the planet.

The impact sends dust and
ash high into the atmosphere

And the climate becomes colder and drier,

Particularly-in the northern hemisphere.

[narrator] incredibly,
some abu hureyra people

Survive the apocalypse.

But climate change
turns their garden of eden

Into dry scrubland.

Their extraordinary ability to adapt

Would have far-reaching implications.

Astonishingly, barley
and rye remains are found

Above the layer of destruction.

We also find stone tools

That were being used
to grind cereal grains.

This evidence suggests that
the inhabitants of abu hureyra

Began the first methods of farming

To replace the plants that they
couldn't find out in the wild anymore.

What we see is one of the
most profound alterations

In the course of human history.

This move to systematic farming

Was a huge leap forward in the
way we organized our societies

In the ancient past.

It is the building blocks
for complex civilizations

Such as we know today.

[narrator] the andes mountains, peru.

Almost 8,000 feet above sea-level,

Sits one of the new
seven wonders of the world.

The legendary machu picchu.

Machu picchu is in the middle
of a tropical mountain forest.

It's the most amazing urban
creation of the inca empire.

[koons] it is truly one of the most
astonishing man-made marvels.

The inca had a deep
appreciation of this landscape.

And they designed machu picchu in harmony.

[narrator] for over a century,

Experts have scrutinized the ruins.

But one key question still eludes them.

Why the inca civilization
chose this precarious site

For their sacred citadel.

The terrain is unbelievably jagged.

And there are sheer drops
to the river valley below.

The logistics of building
here are mind-boggling.

It's like trying to build manhattan

In the middle of the rockies.

It just doesn't make any sense.

[narrator] now, digital geoarchaeology

May finally unlock the hidden secret

To this architectural wonder.

We can see the machu
picchu stands right there.

It's uncanny.

X really does mark the spot.

[narrator] machu picchu,

The jewel of the inca empire.

Set over 80,000 acres,

Are more than 200 awe-inspiring buildings

Among dramatic plazas and terraces.

But one mystery continues
to confound experts.

Why build it high

In the inhospitable andes?

Machu picchu was
constructed in the mid-1400s.

This is the time of the
incan ruler pachacuti.

Why pachacuti chose this remote location

Defies logic.

There must be something
exceptional about this site

That made the incas move heaven and earth

To build here.

[narrator] most puzzling,

Is this environment
conspires against construction.

Not only was machu picchu
perched on this mountain top,

It's in an area plagued by earthquakes.

Surely, this is not the
best place to build a city.

[narrator] and altitude adds yet
another baffling layer to the mystery.

The rock here is 250
million-year-old granite.

It's hard. It's heavy. And
it's really difficult to mine.

[rose] transporting thousands
of these granite blocks

Up from the valley floor
or quarrying them up high

Seems unbelievable.

[narrator] now it's hoped, geoarchaeology

Applying earth science to historic sites,

Can crack this machu picchu conundrum.

Geological survey data is
used to create a 3d model.

This is loaded into the tech lab,

Ready for historian
sascha auerbach to analyze.

We created this detailed digital model

Of the machu picchu area.

Here we have the citadel.

And on either side, we've got the peaks,

Here and here.

So, the andes are renowned
for their tectonic activity.

It's crisscrossed with fault lines

And volcanos belch everywhere.

So, first, let us plot
some of those fault lines.

Here we can see three of those fault lines.

Machu picchu is built along
this one, marked in yellow.

And it's flanked by two
others, these white lines,

That are roughly parallel.

Now, between these two,

A chunk of land called
a graben has collapsed.

And this has had an interesting effect.

It's formed a dip between these two peaks.

More interestingly,
its created flatter land

On which you can build the citadel.

Geologists have plotted
the major andes fault lines

On a grand scale.

And this reveals something else.

If I zoom in,

We can see that machu
picchu stand right there

At the intersection of three
of these major fault lines.

It's uncanny. X really does mark the spot.

I think what we're seeing is a
crucial piece of a jigsaw puzzle

To explain why the incas built machu picchu

Right here.

[narrator] this positioning
seems beyond coincidence.

Further investigation reveals
the inca were savvy enough

To embrace this volatile
tectonic crossroads.

[proctor] the incas had a deep
appreciation of the landscape.

Where multiple fault lines meet,
the stones are more shattered,

Forming irregular shapes like triangles
and hexagons and rhombohedrals.

These are the exact same stones

The inca masons are
using to build machu picchu.

It's amazing that the
inca were handed these

Naturally quarried stone blocks on a plate.

The rocks naturally fracture
along planes of weakness.

It's easy to quarry following these lines.

And the granite has a
high crystalline structure

Which makes it very useful

For high tolerance carving.

[narrator] this enables the
inca to intricately assemble

Impressive structures,

Some using blocks weighing over 50 tons.

The walls of the citadel
are an absolute work of art.

The rocks fit together with such precision

That you can't even fit a
credit card between them.

And the inca, they didn't even use mortar.

[schwartz] the inca built the stones

To intricately interlock.

There's actually one stone

That has 30 precise angles to it.

[narrator] this masterful
dry stone construction

Is at the core of machu
picchu's astonishing longevity.

[proctor] they built the
walls with thick bases

To add sturdiness.

And there's no spaces within the cracks.

So, when the tremors hit,

The rocks kinda dance in position

And then settle back in place.

It's a master build,

Making this place earthquake proof.

[narrator] and setting up a
citadel in an earthquake hot zone

Confers one final crucial benefit.

This jungly area

Suffers torrential
rainstorms and flash flooding.

But the system of faults

Prevents machu picchu from
being washed off the mountain.

These deep cracks in the rocks

Efficiently drain excess water away.

Overall, the fault lines in machu picchu

Prove to be a blessing and not a curse.

[narrator] machu picchu is
considered the incas greatest legacy.

Now, earth science shows
how their innate ingenuity

Can harness nature.

The inca demonstrate an
incredible harmony with the land.

Choosing this inhospitable location

And turning it into this
mountain top paradise.

It is reckoned to be the
most amazing creation

Of the inca empire at its height.

[narrator] jersey, the british isles.

2012.

Metal detectorists on the island

Strike upon the find of a lifetime.

Buried just three feet below the surface,

Is a huge pile of green coins,

That are all fused together.

This is a stunning find.

The size of this hoard
is truly extraordinary.

No one has ever found
anything on this scale before.

[bellinger] this is real
life buried treasure.

For jersey, it's as if they had found

The tomb of tutankhamun on their island.

I mean, it's that big a deal.

[narrator] this is the largest
iron age coin haul found on earth

Worth millions of dollars today.

When deposited, it was a king's ransom.

Yet, unbelievably, it was left abandoned.

This is an incredibly valuable hoard.

So, who are the owners

And why didn't they come back for it?

There must be a crazy story here.

If we can work it out.

[narrator] archeologists
are hunting for the owners

Of huge stash of iron age coins

Found on the english
channel island, jersey.

Amazingly, this
once-in-a-lifetime discovery

Was 30 years in the making.

Starting with a young girl.

[rose] people say a farmer's daughter

Was given some ancient
coins dug up by her father.

Not knowing what they were,
she swapped them for comic books.

Locals have always wondered

If these coins were part of a buried hoard

Hidden somewhere on this island.

[narrator] this rumor inspired
two amateur metal detectorists.

After decades scouring farmers' fields,

In 2012, they hit pay dirt.

The coins were found in this lump.

And the whole thing was so compacted

That they couldn't
take it apart in the field.

Um, so, the approach was
to block-lift the whole lot,

Um, and take it to a laboratory.

[narrator] the mud-caked coin block

Weighs over a ton.

Being underground for centuries

Complicates identifying the mystery owner.

Copper corrodes when it's exposed to oxygen

Or water molecules in the ground.

This process fuses the old coins together

And creates a green film covering
the intricate designs on the coins.

[narrator] it takes almost three
years to unpick nearly 70,000 coins.

Each one's location is
recorded by laser probe.

While a laser scanner captures
individual surface details.

This laser is so precise

That it picks out intricate
details on each of the coins.

Such as a horse And the head of a man,

Which is crucial information.

The surface images and the
metallic make-up are like an id card,

Telling us that most coins
are from a powerful celtic tribe

Called the curiosolitae.

[narrator] metallurgical analysis
identifies the coins are made of billon,

An alloy of copper and silver

Consistent with coriosolite minting.

[horton] so the coriosolite are
the tribe living in northern France.

We also know from caesar's gaelic wars,

Um, that they were a bit of a
thorn in the side of the romans.

And the roman campaigns against them

Between 58 and 53 bc

Is well documented by caesar.

[men yelling]

But also well known, in terms of hill forts

That are located along the
northern french coastline.

[narrator] science and
old-fashioned detective work,

Have traced the owners of this
unprecedented iron age coin haul.

But one baffling mystery persists.

This collection of coins would've been
extremely valuable to the curiosolitae.

What would force this tribe to
make this dangerous passage

With this precious cargo
to bury it on the island

And never come back?

[narrator] more perplexing,

The coriosolite also relinquished
revered iron age symbols

Of wealth, power and connections.

[bellinger] within the coin pile

Are precious golden hoop neck-rings

Known as torcs.

Torcs were reserved for the
highest level of celtic society.

And elites would wear
them in important rituals.

They would even wear them into battles,

Such that it became associated,

Uh, inevitably, with the warrior class.

[horton] torcs are
highly prestigious items.

So, this kind of raises the
question of "what's going on?"

[narrator] it's a question that
continues to confound experts worldwide.

No one can agree why the coriosolite

Would inexplicably abandon
their treasure to the earth.

And other iron age coin
discoveries on jersey

Muddy the waters further.

The conventional
interpretation is essentially that

Taking the tribal treasure and
putting it on jersey for safe-keeping

Out of the reach of caesar's armies.

The other explanation,

Is that evidence of metallurgy
and craft activity on the site

Suggest the wealth of
the tribe is being brought,

Um, to be reworked, To be reminted.

But why did they never actually
recover the hoard afterwards?

The whole thing remains a bit of a mystery.

There are a number of
possibilities, to be honest.

All we can hope is that future research

Will help us uncover the truth, eventually.

[narrator] teposcolula-yucundaa, mexico.

2004.

Archeologists investigating
an historic mixtec settlement

Make a series of puzzling finds.

The most striking thing
about teposcolula-yucundaa,

Is that the mixtec have
dropped everything to leave town.

[narrator] chilling evidence
of what caused them to flee

Is found in the town's central plaza.

They find these oval-shaped holes

That have been cut into
this really ornate plaster floor.

And inside are human remains.

It was as if they would open a hole

And stuff it with as
many bodies as possible.

What is particularly mysterious
is that this type of burial

Doesn't follow any of the
traditional mixtec forms.

[narrator] records reveal
teposcolula-yucundaa

Was abandoned in the mid-16th century,

Right at the time an unknown plague

Is ripping through mesoamerica.

The locals and the spanish had never seen

A disease like this.

It was the ultimate,
sort of, mystery killer.

This endemic is estimated to have wiped out

Up to 80% of the central
american population.

That's even more than the black death

That ravaged the european
populations in the middle ages.

[narrator] over the centuries,

No one has been able to pin down the cause

Of this killer plague.

Now, it's hoped the latest
in digital genome analysis

Can finally unlock its secrets.

The cemetery may hold the bodies

From one of the worst
epidemics in human history.

[king] the question is,
what is this lethal killer?

And how is it spreading like wildfire,

Killing the local population?

[narrator] the abandoned mixtec
town, teposcolula-yucundaa,

Is rumored to be ground-zero

For a mystery 16th century plague.

The finger of blame
pointed to spanish invaders.

They arrive in mesoamerica in 1519

On a brutal pillage for gold.

The native population had zero chance

Against these conquistadors.

Not only because of
their weapons, their guns,

But also because of their germs.

The immune systems of the
natives just couldn't withstand them.

This led to one of the most
horrific one-sided assaults

Of a people ever seen in history.

[narrator] smallpox,
measles, influenza and malaria

Decimate native mesoamericans.

But one invisible killer
defies explanation.

The locals referred to it as cocoliztli,

Great pestilence.

It was a disease that resulted

In severe abdominal pains, fevers,

And bleeding from the eyes and the mouth.

Death usually resulted in a few days.

One spanish priest

States that he buried over
10,000 people in a single year.

To put that in perspective,

There were scores of
spanish priests in that area.

[king] was this something
that's been brought over

From europe with the spaniards?

Or is it some hitherto unknown,

Sort of, killer disease
from deep in the jungle?

[narrator] scientist believe that
teposcolula-yucundaa plague pits

May provide the vital missing link.

They start by scouring the
human remains for pathogens.

Using polymerase chain reaction or pcr.

Polymerase chain reaction is a technique

Which means we can use a tiny bit of dna

And it can be replicated
over and over again.

Billions of times.

And then it can be examined.

In this case, polymerase chain reaction

Can be used to examine an ancient pathogen,

Something that caused
this terrible disease.

[narrator] but the teams
best efforts are thwarted.

Millions of dna molecules
from sources like fungi,

Plants, insects and animals,

Severely cloud the results.

To combat this, focus is
switched to the victim's inner teeth.

Scientists reckon that the
best way of looking at the dna

Of a blood-borne pathogen,
is to look at the tooth pulp.

Because the tooth enamel will protect that

From external contaminants.

After looking at the dna samples,

There are several thousand
different bacterial species.

Finding the one that may have
been the killer is a daunting prospect.

To crack this case, they need
a large-scale screening process.

[narrator] this crucial
breakthrough comes in 2016

Via a new generation
of bio-informatics tech.

The megan alignment tool or malt.

So, what's really, really cool

Is that it has completely
revolutionized ancient dna research.

What scientists do is they take

All of the strands of dna
that have been sequenced

And use a computer program

To, basically, say, "right, where
is this dna sequence from?"

And match it against databases of known

Modern, uh, bacterial dna sequences.

Essentially, sifting through
all the information for you

To find tiny bits of evidence of pathogens

That were previously hidden to us.

[narrator] malt crunches the
data from these new samples,

Screening for any
hint of a killer pathogen.

The sequencing discounts
the usual suspects.

Smallpox, measles, flu.

But it does land on one suspect.

In a few teeth, they find pieces of dna

From salmonella enterica.

The cause for enteric fever.

[narrator] crucially, enteric fever
matches many of the symptoms

Described during the cocoliztli epidemic.

Until this discovery among these skeletons,

Nothing was actually
known about the prevalence

Of this particular pathogen

In the past and among these people.

Cutting-edge science has
now been able to identify

The bacteria that was
part of this huge epidemic

And decline in this population.

[narrator] but one mystery remains.

How did this deadly pathogen flare up

In 16th century mesoamerica?

Scientists run the same tests on burials

That pre-date european contact

And find no example of enteric fever.

The blame for this disease falls
squarely on spanish colonizers.

It's thought that the europeans
carried the bug to the americas

Through livestock or
through human carriers.

Once in the americas,

It would leech into the
food and water supplies

Through vomit and fecal
material of sick individuals.

It would then spread and ultimately kill

Millions of the central
american population.

[narrator] having cracked
this centuries-old case

At teposcolula-yucundaa,

Next generation genomic analysis

Is being touted as the pathogen pioneer,

A crucial bio-informatics tool

For unlocking our hidden
disease-ravaged past.

[king] many historic diseases
were absolutely catastrophic.

I mean, they collapsed cities.

Entire populations were decimated.

But now, with this cutting-edge science,

We can understand more about
how these deadly pathogens

Have shaped human history.